Transcription factors NF-YB involved in embryogenesis and hormones responses in Dimocarpus Longan Lour.

Introduction: NF-YB transcription factor is an important regulatory factor in plant embryonic development. Results: In this study, 15 longan NF-YB (DlNF-YB) family genes were systematically identified in the whole genome of longan, and a comprehensive bioinformatics analysis of DlNF-YB family was performed. Comparative transcriptome analysis of DlNF-YBs expression in different tissues, early somatic embryogenesis (SE), and under different light and temperature treatments revealed its specific expression profiles and potential biological functions in longan SE. The qRT-PCR results implied that the expression patterns of DlNF-YBs were different during SE and the zygotic embryo development of longan. Supplementary 2,4-D, NPA, and PP333 in longan EC notably inhibited the expression of DlNF-YBs; ABA, IAA, and GA3 suppressed the expressions of DlNF-YB6 and DlNF-YB9, but IAA and GA3 induced the other DlNF-YBs. Subcellular localization indicated that DlNF-YB6 and DlNF-YB9 were located in the nucleus. Furthermore, verification by the modified 5'RNA Ligase Mediated Rapid Amplification of cDNA Ends (5' RLM-RACE) method demonstrated that DlNF-YB6 was targeted by dlo-miR2118e, and dlo-miR2118e regulated longan somatic embryogenesis (SE) by targeting DlNF-YB6. Compared with CaMV35S- actuated GUS expression, DlNF-YB6 and DlNF-YB9 promoters significantly drove GUS expression. Meanwhile, promoter activities were induced to the highest by GA3 but suppressed by IAA. ABA induced the activities of the promoter of DlNF-YB9, whereas it inhibited the promoter of DlNF-YB6. Discussion: Hence, DlNF-YB might play a prominent role in longan somatic and zygotic embryo development, and it is involved in complex plant hormones signaling pathways.

Genome-Wide Identification and Expression Analysis Reveals the B3 Superfamily Involved in Embryogenesis and Hormone Responses in Dimocarpus longan Lour.

B3 family transcription factors play an essential regulatory role in plant growth and development processes. This study performed a comprehensive analysis of the B3 family transcription factor in longan (Dimocarpus longan Lour.), and a total of 75 DlB3 genes were identified. DlB3 genes were unevenly distributed on the 15 chromosomes of longan. Based on the protein domain similarities and functional diversities, the DlB3 family was further clustered into four subgroups (ARF, RAV, LAV, and REM). Bioinformatics and comparative analyses of B3 superfamily expression were conducted in different light and with different temperatures and tissues, and early somatic embryogenesis (SE) revealed its specific expression profile and potential biological functions during longan early SE. The qRT-PCR results indicated that DlB3 family members played a crucial role in longan SE and zygotic embryo development. Exogenous treatments of 2,4-D (2,4-dichlorophenoxyacetic acid), NPA (N-1-naphthylphthalamic acid), and PP333 (paclobutrazol) could significantly inhibit the expression of the DlB3 family. Supplementary ABA (abscisic acid), IAA (indole-3-acetic acid), and GA3 (gibberellin) suppressed the expressions of DlLEC2, DlARF16, DlTEM1, DlVAL2, and DlREM40, but DlFUS3, DlARF5, and DlREM9 showed an opposite trend. Furthermore, subcellular localization indicated that DlLEC2 and DlFUS3 were located in the nucleus, suggesting that they played a role in the nucleus. Therefore, DlB3s might be involved in complex plant hormone signal transduction pathways during longan SE and zygotic embryo development.

The distorted power of medical surgical masks for changing the human thermal psychology of indoor personnel in summer.

The medical surgical mask (MSM) has been the essential protective equipment in people's daily work. The experimental purpose is to explore the effects of wearing MSM on human thermal sensation, thermal comfort, and breathing comfort in office buildings in summer. A total of 30 healthy college students were recruited for the testing. The experiment was carried out in a climate chamber, which can simulate the office buildings in summer. The experiment collects the subjects' skin temperature, microclimate in the mask, and subjective votes, including thermal sensory votes (TSV), thermal comfort votes (TCV), and respiratory comfort votes (BCV). Experimental results show that wearing MSM has no significant effect on the skin temperature of the human body. The microclimate temperature inside the MSM reaches over 34℃, and the relative humidity reaches over 70%. The high-temperature and high-humidity microclimate put human beings in an uneven thermal environment, which leads to poor human tolerance to the thermal environment and becomes the main reason for destroying human thermal comfort. Wearing MSM has a significant impact on the subjective thermal sensation, thermal comfort, and breathing comfort of the human body, and the impact becomes more significant as the environmental temperature increases. Once the mask is taken off, the human body will enter an extremely comfortable environment, resulting in an excessively high vote value. The difference in voting values before and after removing the mask becomes larger with the environmental temperature. By fitting the voting results and perform data processing, it can be found that wearing MSM will reduce the neutral temperature by 1.5°C, and the environmental temperature with the optimal thermal comfort by 1.4°C, and as the temperature increases, the respiratory discomfort will become more and more intense. Regardless of whether wearing a MSM, the subjects preferred a slight warmer environment. In conclusion, with the increase of ambient temperature, wearing MSM can cause the human worse tolerance to the thermal environment, and this disturbance will become more and more intense.